Rabbit polyclonal antibody to PU.1 (R212)

The Ets transcription factor family (Ets~1, Ets~2, Erg~1–3, Elk~1, Elf~1, Elf~5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli~1, TEL, Spi~B, ESE~1, ESE~3A, Net, ABT1 and ERF) are DNA~binding proteins that influence lymphoid development and activity. The Ets family monomeric proteins bind the consensus DNA site GGA(A/T) through a unique winged helix~turn~helix motif known as the Ets domain. PU.1 (Spi~1/Spi~A), Spi~B and Spi~C are closely related Ets family members which share a conserved divergent sequence within the Ets domain that enables their binding to the non~canonical AGAA sites. PU.1 transactivates a large number of B cell genes, such as those encoding CD72, CD20 and Btk, and Spi~B enhances expression of many of these same target genes. PU.1 is expressed in a wide variety of hematopoetic cells, including B cells, early T cells, megakaryocytes, granulocytes, mast cells, immature erythrocytes and myeloid cells. Alternatively, Spi~B expression is limited to B cells and immature T cells, where expression accumulates through T lineage commitment and then is dramatically absent following the β selection checkpoint.