Rabbit polyclonal antibody to DAP~5 (N65)

Death~associated protein 5 (DAP~5) (also known as p97 and NAT1) is a member of the eukaryotic translation initiation factor 4G (elF4G) family. DAP~5 is ubiquitously expressed and is highly conserved among species. In response to activated FAS or p53, caspase cleaves DAP~5 at position 790 to yield a C~terminal truncated protein which is capable of forming complexes with eIF4A and eIF3. DAP~5 has homology to the carboxy~terminal portion of eIF4G, but lacks the N~terminal region of eIF4G, which is responsible for association with the CAP binding protein eIF4E. By forming translationally inactive complexes with eIF4A and eIF3, but not with eIF4E, DAP~5 functions as a general repressor of translation. During apotosis, the caspase~activated DAP~5 can mediate CAP~independent translation at least from its own internal ribosome entry site, thus resulting in a positive feedback loop responsible for the continuous translation of DAP~5. DAP~5 is also required for cellular differentiation, as it controls specific gene expression pathways.