Rabbit polyclonal antibody to AATF (N16)

Che~1 (protein AATF, apoptosis~antagonizing transcription factor) is a widely expressed nuclear protein that belongs to the AATF family. Hyperphosphorylated during the G1/S phase transition, Che~1 may function as a general inhibitor of the histone deacetylase HDAC1. Che~1 binding to the pocket region of Rb may displace HDAC1 from Rb/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Displacement of HDAC1 from Sp1 bound to the p21 promoter leads to increased expression of Che~1. It also antagonizes PAR4 (prostate apoptosis response 4) mediated induction of aberrant amyloid peptide production in Alzheimer disease (AD; presenile and senile dementia). PAR4 is a leucine zipper protein that is pro~apoptotic and associated with neuronal degeneration in AD. Che~1 interaction with PAR4 suggests that it might directly or indirectly participate in regulation of PAR4 activity. Che~1 also co~localizes with PAR4 in both cytoplasmic and nuclear compartments, and it interacts directly and selectively with PAR4 via the leucine zipper domain in neural cells.