Rabbit polyclonal antibody to TCF~1 (F26)

T~cell factor~1 (TCF~1) is a DNA~binding transcriptional activator that isessential for lymphoid cell development. The TCF family of transcription factors are activated by the Wnt~1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. Several alternative splice variants of TCF~1 have been identified, including TCF~1A, which share a conserved amino terminus and differ in the carboxy terminal sequences. The Wnt mediated signaling pathway induces cytosolic β~catenin binding to TCF proteins within the nucleus, leading to the enhanced expression of the Wnt target genes. The β~catenin~TCF complexes are negatively regulated by the adenomatous polyposis coli (APC) tumor suppressor protein, which phosphorylates β~catenin and, in turn, increases the degradation of cytosolic β~catenin and inhibits the transcriptional activity of the TCF proteins.