Rabbit polyclonal antibody to Raf~1 (Q39)

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf~1. Raf~1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v~Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v~Raf protein has been associated with transformation and mitogenesis, while the activity of Raf~1 is normally suppressed by a regulatory N~terminal domain. Raf~A, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf~1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf~B is highly restricted, with highest levels in the cerebrum and testis.