Rabbit polyclonal antibody to p40~phox (R149)

Nicotinamide adenine dinucleotide phosphate (NADPH)~oxidase is a multimeric enzyme system that mediates electron transport from NADPH in the cytoplasm to molecular oxygen in the phagosome, thereby generating reactive oxidant intermediates. Upon neutrophil stimulation, NADPH~oxidase and other cytosolic elements localize to the cell membrane from the cytosol to form a complex which produces phagocytic oxygen radicals. There are a number of cytosolic proteins that are involved in NADPH~oxidase activation/deactivation,including p47~phox, p67~phox, p40~phox and the small GTP~binding protein, Rac. Activation of NADPH oxidase is accompanied by the phosphorylation of cytosolic components p40~phox, p47~phox and p67~phox. The PKC consensus phosphorylation sites Thr 154 and Ser 315 in p40~phox are phosphorylated during activation of NADPH oxidase. p40~phox can promote oxidase activation by increasing the affinity of p47~phox for NADPH~oxidase.