Rabbit polyclonal antibody to EEF2 (A50)

Two elongation factors (EF) EF~Tu and EF~2 participate in the elongation phase during protein biosynthesis on the ribosome and their functional cycles depend on GTP binding and its hydrolysis. EF~Tu (also designated mitochondrial precursor p43) and EF~2 are multidomain GTPases with essential functions in translation, and they both bind to the same site on the ribosome where their low intrinsic GTPase activities are strongly stimulated. EF~Tu plays a central role in the fast and accurate delivery of aminoacyl~tRNAs to the translating ribosome. In addition, EF~Tu protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anticodon on tRNA can be achieved. EF~2 supports the translocation of tRNAs and of mRNAs on the ribosome so that a new codon can be exposed for decoding.