Rabbit polyclonal antibody to CUL2 (I726)

Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)~mediated proteolysis. CUL~1, which is the mammalian homolog of Cdc53 from yeast, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF(Skp1/CUL~1/F~box protein complex) consists of Skp1 associating with both CUL~1 and an F~box protein, such as Skp2, which determines the substrate specificity of the complex. CUL~1 mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL~3 facilitates the degradation of cyclin E independent of SCF activity, while CUL~2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL~4 associating with Nedd~8, a ubiquitin~ like protein, where it too functions as an active component of a multifunctional E3 complex. CUL~5, or vasopressin~activated, calcium~mobilizing protein (VACM~1), is also included in the cullin family as it shares substantial sequence homology with CUL~1.