Rabbit polyclonal antibody to Cot (P284)

The role of mitogen~activated protein kinases (MAPKs) in cell signaling pathways is well established. The rat gene Tpl~2, for tumor progression locus 2, and the human and mouse homologues c~Cot, for cancer osaka thyroid oncogene, encode a proto~oncogene serine/threonine protein kinase that was shown to play a role in the functional activation of the MAP kinase pathway. Overexpression of Cot induces MAP kinase activation in COS~1 and NIH/3T3 cells. Cot~mediated activation of MAP kinase is inhibited by both Ras N17, a dominant negative mutant of c~H~Ras, and Raf~1s621A, a dominant negative mutant of Raf~1, suggesting that Cot functions upstream of Ras and Raf~1.Other studies have shown that a kinase~negative, dominant negative mutant of Cot partially blocks Ras or Raf~1~induced MAP kinase activation, arguing that Cot functions downstream of Ras and Raf~1.