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Western blot (WB) analysis of Akt polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug)

Immunoprecipitation of A549 cell lysates using AKT pAb (Sepharose Bead Conjugate) (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) (lane 4). Lane 1 is 30% input. The western blot was probed using AKT pAb.

Immunohistochemistry (IHC) analyzes of AKT pAb in paraffin~embedded human kidney carcinoma tissue at 1:50,showing cytoplasm and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti~Rabbit IgG~biotin followed by avidin~peroxidase.

Rabbit polyclonal antibody to AKT

货号：
53009
别名：
RAC~alpha serine/threonine~protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto~oncogene c~Akt; RAC~PK~alpha; AKT1; PKB; RAC; PKBα; RAC~beta serine/threonine~protein kinase; Protein kinase Akt~2; Protein kinase B beta; PKB beta; RAC~PK~beta; AKT2; PKBβ; RAC~gamma serine/threonine~protein kinase; Protein kinase Akt~3; Protein kinase B gamma; PKB gamma; RAC~PK~gamma; STK~2; AKT3; PKBG; PKBγ
产品上架时间：
2024-01-01
应用：
WB IHC IF IP
反应种属：
Human,Mouse,Rat
抗体类型：
Primary antibody
Swissprot：
P31749/P31751/Q9Y243
规格：

50ul
100ul
数量：

-

+
说明书：

目录价¥1280

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Rabbit polyclonal antibody to AKT

Description
AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.

Description

AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.

Specification
Aliases	RAC~alpha serine/threonine~protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto~oncogene c~Akt; RAC~PK~alpha; AKT1; PKB; RAC; PKBα; RAC~beta serine/threonine~protein kinase; Protein kinase Akt~2; Protein kinase B beta; PKB beta; RAC~PK~beta; AKT2; PKBβ; RAC~gamma serine/threonine~protein kinase; Protein kinase Akt~3; Protein kinase B gamma; PKB gamma; RAC~PK~gamma; STK~2; AKT3; PKBG; PKBγ
Swissprot	P31749/P31751/Q9Y243
clone	2A10C5
WB Predicted band size	~60kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at ~20°C long term. Avoid freeze~thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Recombinant full length Human AKT1.
Formulation	1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2