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Western blot analysis of lysates from A375
cells, using MiTF Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.
Immunohistochemical analysis of paraffin-embedded human melanoma tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Rabbit Monoclonal Antibody to MiTF
-
货号：
50539 -
别名：
MITF;BHLHE32;Microphthalmia-associated transcription factor;Class E basic helix-loop-helix protein 32;bHLHe32 -
应用：
WB,IHC,IF,IP,ELISA -
反应种属：
Human,Mouse,Rat, -
抗体类型：
Primary antibody -
Swissprot：
O75030 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal Antibody to MiTF
Description |
|---|
This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008], |
Specification |
|
|---|---|
| Aliases | MITF;BHLHE32;Microphthalmia-associated transcription factor;Class E basic helix-loop-helix protein 32;bHLHe32 |
| Conjugation | Unmodified |
| Entrez GeneID | 4286 |
| Swissprot | O75030 |
| WB Predicted band size | 58kD |
| Host/Isotype | Rabbit IgG,Kappa |
| Antibody Type | Primary antibody |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Species Reactivity | Human,Mouse,Rat, |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
Application |
|
|---|---|
| WB | 1/1000-5000 |
| IP | 1/50-200 |
| IHC | 1/200-500 |
| IF/ICC | 1/200-1000 |
| ELISA | 1/5000-20000 |
Product Image
- Western blot analysis of lysates from A375 cells, using MiTF Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.
- Immunohistochemical analysis of paraffin-embedded human melanoma tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
- Immunohistochemical analysis of paraffin-embedded mouse placenta tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
- Immunohistochemical analysis of paraffin-embedded mouse placenta tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#50539
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