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Western blot analysis of ATG3 in K562, rat Brain, C6, 3T3, Hela lysates using ATG3 antibody.
Immunocytochemistry analysis of ATG3(green) in Hela using ATG3 antibody,and DAPI(blue)
Immunohistochemistry analysis of paraffin-embedded Human tonsil using ATG3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Rabbit Monoclonal Antibody to ATG3

  • 货号:

    P21090
  • 别名:

    APG3; APG3L; PC3-96; APG3-LIKE
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,IF
  • 反应种属:

    Human,Mouse,Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q9NT62
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Monoclonal Antibody to ATG3

Description

E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). Catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.

Specification

Aliases APG3; APG3L; PC3-96; APG3-LIKE
Entrez GeneID 64422
Swissprot Q9NT62
WB Predicted band size Calculated MW: 36 kDa; Observed MW: 40 kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat
Immunogen A synthetic peptide of human ATG3
Formulation Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

Application

WB 1/500-1/1000
IHC 1/50-1/100
IF 1/50-1/200