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Western blot analysis of APPBP1 in SKOV-3 lysates using APPBP1 antibody.
HOT
Rabbit Monoclonal Antibody to APPBP1
-
货号：
P21065 -
别名：
NEDD8 activating enzyme E1 subunit 1; HPP1; ula-1; APPBP1; A-116A10.1 -
应用：
WB,IP -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q13564 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal Antibody to APPBP1
Description |
|---|
Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. |
Specification |
|
|---|---|
| Aliases | NEDD8 activating enzyme E1 subunit 1; HPP1; ula-1; APPBP1; A-116A10.1 |
| Entrez GeneID | 8883 |
| Swissprot | Q13564 |
| WB Predicted band size | Calculated MW: 60 kDa; Observed MW: 60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human APPBP1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IP | 1/10-1/20 |
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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