请登录
搜索 英文站
栏目

提醒成功

请输入手机号/邮箱号
请输入密码

账号密码登录

微信/QQ登录 微信/QQ登录

没有账号? 立即注册

搜索
Red: Control Antigen (100ng) Purple: Antigen (10ng), Green: Antigen (50ng) Blue: Antigen (100ng),
Western blot analysis using His tag mAb against recombinant protein. lane 1 :(100 ng); lane 2 :(50 ng); lane 3 :(25 ng); lane 4 :(10 ng); lane 5 :(2.5 ng); (Expected MW is 50 kDa).
HOT

Mouse Monoclonal Antibody to His Tag

  • 货号:

    32442
  • 别名:

    6 His tag; HHHHHH tag
  • 产品上架时间:

    2024-04-01
  • 应用:

    WB,IP,IHC,IF,FCM
  • 反应种属:

    Tag
  • 抗体类型:

    Tag Antibody
  • Swissprot:

  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1320

加入购物车

Mouse Monoclonal Antibody to His Tag

Description

The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.

Specification

Aliases 6 His tag; HHHHHH tag
clone 6A8H8
Host/Isotype Mouse IgG1
Antibody Type Tag Antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Tag
Immunogen Synthesized peptide of His Tag (AA: HHHHHHHH-KLH).
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500-1/1000
IP 1/20-1/200
IHC 1/100 - 1/1000
IF 1/100 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#32442