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Western blot analysis of IDH3 alpha expression in MCF7, HT29, mouse brain, mouse kidney whole cell lysates.
Immunofluorescent analysis of GPRC5D staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Polyclonal Antibody to GPRC5D
-
货号：
P43699 -
别名：
G-protein coupled receptor family C group 5 member D -
应用：
WB,IF -
反应种属：
Human,Mouse,Rat -
抗体类型：
Primary antibody -
Swissprot：
Q9NZD1 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to GPRC5D
Specification |
|
|---|---|
| Aliases | G-protein coupled receptor family C group 5 member D |
| Entrez GeneID | 55507; |
| Swissprot | Q9NZD1 |
| WB Predicted band size | 39kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPRC5D. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IF | 1/100-1/300 |
Product Image
- Western blot analysis of IDH3 alpha expression in MCF7, HT29, mouse brain, mouse kidney whole cell lysates.
- Immunofluorescent analysis of GPRC5D staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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