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Western blot analysis using EI2BG mouse mAb against K562 (1), Hela (2),MCF-7 (3) and HL-60 (4) cell lysate.
Immunofluorescence analysis of Hela cells using EI2BG mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of HL-60 cells using EI2BG mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human liver tissues using EI2BG mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human kidney tissues using EI2BG mouse mAb with DAB staining.
Mouse Monoclonal Antibody to EI2BG
-
货号：
32387 -
别名：
EIF2B3;EIF-2B; EIF2Bgamma -
应用：
WB,IHC,IF,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q9NR50 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to EI2BG
Description |
|---|
The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
References |
|---|
| 1.Brain Dev. 2016 May;38(5):507-15. 2.PLoS One. 2015 Mar 11;10(3):e0118001. |
Specification |
|
|---|---|
| Aliases | EIF2B3;EIF-2B; EIF2Bgamma |
| Entrez GeneID | 8891 |
| Swissprot | Q9NR50 |
| clone | 1B9C12 |
| WB Predicted band size | 50.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human EI2BG expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| IF/ICC | 1/50 - 1/200 |
| FCM | 1/200 - 1/400 |
Product Image
- Western blot analysis using EI2BG mouse mAb against K562 (1), Hela (2),MCF-7 (3) and HL-60 (4) cell lysate.
- Immunofluorescence analysis of Hela cells using EI2BG mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of HL-60 cells using EI2BG mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human liver tissues using EI2BG mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human kidney tissues using EI2BG mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#32387
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