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Western blot analysis using GPI mouse mAb against HepG2 (1) , SMMC-7721 (2) cell lysate and rat liver tissues lysate (3).
Confocal Immunofluorescence analysis of L-02 cells using GPI mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemical analysis of paraffin-embedded human Kidney tissues using GPI mouse mAb.
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Mouse Monoclonal Antibody to Glucose-6-phosphate isomerase

  • 货号:

    20198
  • 别名:

    AMF; NLK; PGI; PHI; GNPI; SA-36; GPI
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,IF
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P06744
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to Glucose-6-phosphate isomerase

Description

Glucose-6-phosphate isomerase, or phosphoglucose isomerase, also known as GPI. It belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways and it is an enzyme that catalyzes the conversion of glucose-6-phosphate into fructose 6-phosphate in the second step of glycolysis. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in GPI are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

References

1. Biochem Biophys Res Commun. 2004 Oct 15;323(2):518-22.
2. Biochem Biophys Res Commun. 2006 Oct 20;349(2):838-45.
3. Hum Mutat. 2006 Nov;27(11):1159.
4. Leuk Lymphoma. 2006 Oct;47(10):2234-43.

Specification

Aliases AMF; NLK; PGI; PHI; GNPI; SA-36; GPI
Entrez GeneID 2821
Swissprot P06744
clone 1B7D7
WB Predicted band size 63kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Purified recombinant fragment of human GPI expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

IHC 1/200 - 1/1000
IF 1/200 - 1/1000
ELISA 1/10000