Rabbit Polyclonal Antibody to eIF4E(Ab-209)

Description
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

References
Li BD, et al. (1998) Ann Surg; 227(5): 756-763Altmann M, et al. (1989) Nucleic Acids Res; 17(18): 7520De Gregorio E, et al. (2001) RNA; 7(1): 106-113Gu W, et al. (2004) Nucleic Acids Res; 32(15): 4448-4461Ohlmann T, et al. (1996) EMBO J; 15(6): 1371-1382

Specification
Aliases	mRNA cap-binding protein; eIF-4F 25 kDa subunit;
Entrez GeneID	1977;
Swissprot	P06730
WB Predicted band size	25kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Peptide sequence around aa. 207~211 (S-G-S-T-T) derived from Human eIF4E.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

Application
WB	1/500-1/1000
IHC	1/50-1/100
IF/ICC	1/100-1/200