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Mouse Monoclonal Antibody to Vimentin
-
货号:
20118 -
别名:
FLJ36605; VIM -
应用:
WB,IHC,FCM -
反应种属:
Human,Mouse,Monkey -
抗体类型:
Primary antibody -
Swissprot:
P08670 -
规格:
-
数量:
-+ -
说明书:
目录价¥2180

Mouse Monoclonal Antibody to Vimentin
Description |
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Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments. |
References |
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1. Seshadri, R., et al. Intl. J. Cancer 67: 353-356(1996) 2. Essa, T.M., et al. J. Egyptian Soc. Parasitol. 26:433-442(1996) 3. Chu, Y.W., et al. Amer.J. Pathol. 148: 63-69(1996) |
Specification |
|
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Aliases | FLJ36605; VIM |
Entrez GeneID | 7431 |
Swissprot | P08670 |
clone | 9E7E7 |
WB Predicted band size | 54kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Monkey |
Immunogen | Purified recombinant fragment of Vimentin expressed in E. Coli. |
Formulation | Ascitic fluid containing 0.03% sodium azide. |
Application |
|
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WB | 1/500 - 1/2000 |
IHC | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
- Western blot analysis using Vimentin mouse mAb against NIH/3T3 cell lysate.
- Flow cytometric analysis of COS7 cells using Vimentin mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing cytoplasmic localization using Vimentin mouse mAb with DAB staining.
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#20118

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