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Rabbit Polyclonal Antibody to 4E-BP1 (Phospho-Ser65)
-
货号:
P40729 -
别名:
4EBP1; EIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Insulin-stimulated EIF-4E binding protein PHAS-I; P/OKCL.6; PHAS-1; PHAS- -
应用:
WB,IHC -
反应种属:
Human,Mouse,Rat -
抗体类型:
Primary antibody -
Swissprot:
Q13541 -
规格:
-
数量:
-+ -
说明书:
目录价¥2180
Rabbit Polyclonal Antibody to 4E-BP1 (Phospho-Ser65)
Description |
|---|
Regulates eIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. |
Specification |
|
|---|---|
| Aliases | 4EBP1; EIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Insulin-stimulated EIF-4E binding protein PHAS-I; P/OKCL.6; PHAS-1; PHAS- |
| Entrez GeneID | 1978; |
| Swissprot | Q13541 |
| WB Predicted band size | 15kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 64 (R-N-S(p)-P-V) derived from Human 4E-BP1. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/3000 |
| IHC | 1/50-1/100 |
Product Image
- Western blot analysis of extracts from Jurkat cells, treated with Insulin (0.01U/ml, 15mins), using 4E-BP1 (Phospho-Ser64) antibody P40729. The lane on the right is treated with the synthesized peptide.
- mmunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 (Phospho-Ser64) antibody. The picture on the right is treated with the synthesized peptide.
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P40729
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