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Western blot analysis using ApoM mouse mAb against human serum (1, 2).
Immunofluorescence analysis of methanol-fixed L-02 (left) and Cos7 (right) cells using ApoM mouse mAb showing cytoplasmic and membrane localization.
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Mouse Monoclonal Antibody to ApoM

  • 货号:

    20116
  • 别名:

    G3a; NG20; HSPC336; MGC22400
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IF
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    O95445
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to ApoM

Description

ApoM (apolipoprotein M, also designated G3a or NG20), with 188-amino acid protein(about 21kDa), is an apolipoprotein and member of the lipocalin protein family. The Apo-proteins are involved in the specific binding of cellular receptors, the regulation of lipolytic enzymes, and the process of lipid exchange. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. The N-terminal region of Apo-M contains hydrophobic residues that may promote association with the phospholipid layer of lipoprotein particles. In vitro, Apo-M is glycosylated when translated in the presence of microsomes, and remains associated with the microsomes after carbonate treatment. Apo-M is expressed in liver and kidney, and is secreted into the bloodstream in HDLs, and also found in triglyceride-rich lipoproteins and LDLs.

References

1. Xu, N. & Dahlback, B. 1999 J. Biol. Chem. 274:31286
2. Duan J, Dahlback B, Villoutreix BO.FEBS Lett. 2001 Jun 15;499(1-2):127-32.
3. Xu,N., Nilsson-Ehle,P. & Ahren,B. 2004. J. Nutr.Biochem. 15 (10):579-582
4. Zhang,X.Y. ,et al.2004. Acta Histochem. 106 (2):123-128

Specification

Aliases G3a; NG20; HSPC336; MGC22400
Entrez GeneID 55937
Swissprot O95445
clone 8F12C6B8
WB Predicted band size 21kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Purified recombinant fragment of human ApoM expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/500 - 1/2000
IF 1/200 - 1/1000
ELISA 1/10000

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#20116