Rabbit Polyclonal Antibody to Caspase 9 (Phospho-Thr125)

Description
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

References
Duan H., J. Biol. Chem. 271:16720-16724(1996)Srinivasula S.M., J. Biol. Chem. 271:27099-27106(1996)Hadano S., Mamm. Genome 10:757-760(1999)

Specification
Aliases	APAF-3; MCH6; RNCASP9; CASP-9; ICE-LAP6
Entrez GeneID	842;
Swissprot	P55211
WB Predicted band size	47kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Peptide sequence around phosphorylation site of threonine 125 (P-E-T(p)-P-R) derived from Human Caspase 9.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

Application
WB	1/500-1/1000
IHC	1/50-1/100