Rabbit Polyclonal Antibody to MLC2 (Phospho-Ser15)

Description
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.

References

1) Xia, Y. et al. c-Jun downregulation by HDAC3-dependent transcriptionalrepression promotes osmotic stress-induced cell apoptosis. Mol. Cell 25,219–232 (2007). 2)Vander Heiden, M. G. et al. Evidence for an alternative glycolytic pathway inrapidly proliferating cells. Science 329, 1492–1499 (2010). 3) Fang, D. et al. Phosphorylation of beta-catenin by AKT promotes beta-catenintranscriptional activity. J. Biol. Chem. 282, 11221–11229 (2007).

Specification
Aliases	CMH10, MFM12, MLC-2, MLC-2s/v, MLC-2v, MLC2
Entrez GeneID	4633;
Swissprot	P10916
WB Predicted band size	20kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Peptide sequence around phosphorylation site of serine 15 (A-N-S(p)-N-V) derived from Human MLC2.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

Application
WB	1/500-1/1000
IF	1/100-1/200