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Western blot analysis of Bub3 (Phospho-Tyr207)Antibody P40350 (arrow indicated) in U87 (A) and U87 EGFRvIII (B) cells in Mitosis.

Rabbit Polyclonal Antibody to Bub3 (Phospho-Tyr207)

  • 货号:

    P40350
  • 别名:

    Mitotic checkpoint protein BUB3, Hbub3, BUB3L, BUB3 budding uninhibited by benzimidazoles 3 homolog, budding uninhibited by benomyl
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB
  • 反应种属:

    Human,Mouse,Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    O43684
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Polyclonal Antibody to Bub3 (Phospho-Tyr207)

Description

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.

References

Seeley T.W., Wang L., Zhen J.Y.Biochem. Biophys. Res. Commun. 257:589-595(1999) Tang Z., Shu H., Oncel D., Chen S., Yu H.Mol. Cell 16:387-397(2004) Logarinho E., Resende T., Torres C., Bousbaa H.Mol. Biol. Cell 19:1798-1813(2008)

Specification

Aliases Mitotic checkpoint protein BUB3, Hbub3, BUB3L, BUB3 budding uninhibited by benzimidazoles 3 homolog, budding uninhibited by benomyl
Entrez GeneID 9184;
Swissprot O43684
WB Predicted band size 40kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat
Immunogen Peptide sequence around phosphorylation site of Tyrosine 207 (V-E-Y(p)-L-D) derived from Human Bub3.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/500-1/1000

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#P40350