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Western blot analysis of extracts from HT29 cells untreated or treated with EGF using Paxillin(phospho-Tyr88) Antibody P40322.
Immunofluorescence staining of methanol-fixed Hela cells using Paxillin(phospho-Tyr88) Antibody P40322.
Rabbit Polyclonal Antibody to Paxillin(phospho-Tyr88)
-
货号：
P40322 -
别名：
PAXI; PXN; -
应用：
WB,IF -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
P49023 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Polyclonal Antibody to Paxillin(phospho-Tyr88)
Description |
|---|
An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions |
References |
|---|
| Turner, C.E. (2000) J. Cell Sci. 113, 4139-4140Burridge, K. et al. (1992) J. Cell Biol. 119, 893-903.Bellis, S. L. et al. (1997) Biochem. J. 325, 375-381. |
Specification |
|
|---|---|
| Aliases | PAXI; PXN; |
| Entrez GeneID | 5829; |
| Swissprot | P49023 |
| WB Predicted band size | 68kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 88 (P-V-Y(p)-G-S) derived from Human Paxillin. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IF | 1/100-1/200 |
Product Image
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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