Rabbit Polyclonal Antibody to eIF4B(phospho-Ser422)

Description
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

References
Gingras, A.C. et al. (2001) Genes Dev. 15, 807-826.Duncan, R. and Hershey, J.W. (1985) J. Biol. Chem. 260, 5493-5497.Duncan, R.F. and Hershey, J.W. (1989) J. Cell Biol. 109, 1467-1481.

Specification
Entrez GeneID	1975;
Swissprot	P23588
WB Predicted band size	80kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Peptide sequence around phosphorylation site of Serine 422 (T-G-S(p)-E-S) derived from Human eIF4B.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Application
WB	1/500-1/1000
IHC	1/50-1/100