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Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using Keratin 8(Phospho-Ser74) Antibody P40278.
Western blot analysis of extracts from 293 cells, treated with calf intestinal phosphatase (CIP), using Keratin 8 (Phospho-Ser74) Antibody P40278.
Immunofluorescence staining of methanol-fixed Hela cells using Keratin 8(Phospho-Ser74) Antibody P40278.
Rabbit Polyclonal Antibody to Keratin 8(Phospho-Ser74)
-
货号：
P40278 -
别名：
CK 8; CK8; CYK8; Cytokeratin endo A; K8 -
应用：
WB,IF -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
P05787 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Polyclonal Antibody to Keratin 8(Phospho-Ser74)
Description |
|---|
Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. |
References |
|---|
| Nakamichi I, et al. (2002) Mol Biol Cell; 13(10): 3441-3451. Prochasson P, et al. (2002) Nucleic Acids Res; 30(15): 3312-3322. Pankov R, et al. (1994) Proc Natl Acad Sci U S A; 91(3): 873-877. Thorey IS, et al. (1993) Mol Cell Biol; 13(11): 6742-6751 |
Specification |
|
|---|---|
| Aliases | CK 8; CK8; CYK8; Cytokeratin endo A; K8 |
| Entrez GeneID | 3856; |
| Swissprot | P05787 |
| WB Predicted band size | 55kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 74 (L-L-S(p)-P-L) derived from Human Keratin 8 (CK8). |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IF/ICC | 1/100-1/200 |
Product Image
- Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using Keratin 8(Phospho-Ser74) Antibody P40278.
- Western blot analysis of extracts from 293 cells, treated with calf intestinal phosphatase (CIP), using Keratin 8 (Phospho-Ser74) Antibody P40278.
- Immunofluorescence staining of methanol-fixed Hela cells using Keratin 8(Phospho-Ser74) Antibody P40278.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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