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Western blot analysis of extracts from MCF7 cells using HSF1(phospho-Ser303) Antibody P40243(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using HSF1 (phospho-Ser303) Antibody P40243.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1(Phospho-Ser303) Antibody P40243(left) or the same antibody preincubated with blocking peptide(right).

Rabbit Polyclonal Antibody to HSF1(phospho-Ser303)

  • 货号:

    P40243
  • 别名:

    HSTF 1
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,IF
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q00613
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Polyclonal Antibody to HSF1(phospho-Ser303)

Description

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

References

Parvaneh Rafiee, et,al. (2006) Am J Physiol Cell Physiol ; 291: C931 - C945 Fumika Shinozaki, et,al. (2006) J. Biol. Chem ; 281: 16361 - 16369. Eiichi Takaki, et,al. (2006) J. Biol. Chem ; 281: 4931 - 4937. Jan-Jong Hung, et,al. (1998) J. Biol. Chem ; 273: 31924.

Specification

Aliases HSTF 1
Entrez GeneID 3297;
Swissprot Q00613
WB Predicted band size 82kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Peptide sequence around phosphorylation site of serine 303 (P-P-S(p)-P-P) derived from Human HSF1.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/500-1/1000
IHC 1/50-1/100
IF 1/100-1/200