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Rabbit Polyclonal Antibody to eIF4E(Phospho-Ser209)
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货号:
P40219 -
别名:
mRNA cap-binding protein; eIF-4F 25 kDa subunit; -
应用:
WB,IHC,IF -
反应种属:
Human -
抗体类型:
Primary antibody -
Swissprot:
P06730 -
规格:
-
数量:
-+ -
说明书:
目录价¥2180

Rabbit Polyclonal Antibody to eIF4E(Phospho-Ser209)
Description |
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Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. |
References |
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Li BD, et al. (1998) Ann Surg; 227(5): 756-763 Altmann M, et al. (1989) Nucleic Acids Res; 17(18): 7520 De Gregorio E, et al. (2001) RNA; 7(1): 106-113 Gu W, et al. (2004) Nucleic Acids Res; 32(15): 4448-4461 |
Specification |
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Aliases | mRNA cap-binding protein; eIF-4F 25 kDa subunit; |
Entrez GeneID | 1977; |
Swissprot | P06730 |
WB Predicted band size | 25kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Peptide sequence around phosphorylation site of serine 209 (S-G-S(p)-T-T) derived from Human eIF4E. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
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WB | 1/500-1/1000 |
IHC | 1/50-1/100 |
IF | 1/100-1/200 |
Product Image
- Western blot analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using eIF4E (Phospho-Ser209) Antibody P40219.
- Immunofluorescence staining of methanol-fixed MCF cells using eIF4E(Phospho-Ser209) Antibody P40219.
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E(Phospho-Ser209) Antibody P40219(left) or the same antibody preincubated with blocking peptide(right).
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P40219

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