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Western blot analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using eIF4E (Phospho-Ser209) Antibody P40219.
Immunofluorescence staining of methanol-fixed MCF cells using eIF4E(Phospho-Ser209) Antibody P40219.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E(Phospho-Ser209) Antibody P40219(left) or the same antibody preincubated with blocking peptide(right).

Rabbit Polyclonal Antibody to eIF4E(Phospho-Ser209)

  • 货号:

    P40219
  • 别名:

    mRNA cap-binding protein; eIF-4F 25 kDa subunit;
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,IF
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P06730
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Polyclonal Antibody to eIF4E(Phospho-Ser209)

Description

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

References

Li BD, et al. (1998) Ann Surg; 227(5): 756-763 Altmann M, et al. (1989) Nucleic Acids Res; 17(18): 7520 De Gregorio E, et al. (2001) RNA; 7(1): 106-113 Gu W, et al. (2004) Nucleic Acids Res; 32(15): 4448-4461

Specification

Aliases mRNA cap-binding protein; eIF-4F 25 kDa subunit;
Entrez GeneID 1977;
Swissprot P06730
WB Predicted band size 25kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Peptide sequence around phosphorylation site of serine 209 (S-G-S(p)-T-T) derived from Human eIF4E.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/500-1/1000
IHC 1/50-1/100
IF 1/100-1/200