Rabbit Polyclonal Antibody to MEK1(Phospho-Ser221)

Description
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

References
Zebisch A, et al. (2006) Cancer Res; 66(7): 3401-8. Luciano BS, et al. (2006)J Biol Chem; 279(50): 52117-23. Wang X, et al. (2003) Oncogene; 22(1): 109-16. Gopalbhai K, et al. (2003) J Biol Chem; 278(10): 8118-25. Ling MT, et al. (2002)Oncogene; 21(55): 8498-505.

Specification
Aliases	ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1
Entrez GeneID	5604;
Swissprot	Q02750
WB Predicted band size	45kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Peptide sequence around phosphorylation site of serine 221 (A-N-S(p)-F-V) derived from Human MEK1.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

Application
WB	1/500-1/1000
IHC	1/50-1/100
IF	1/100-1/200