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Western blot analysis of extracts from HT29 cells untreated or treated with PMA using Src(Phospho-Tyr529) Antibody P40148.
Western blot analysis of extracts from HepG2 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using Src (Phospho-Tyr529) Antibody P40148.
Immunofluorescence staining of methanol-fixed Hela cells using Src(Phospho-Tyr529) Antibody P40148.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src(Phospho-Tyr529) Antibody P40148(left) or the same antibody preincubated with blocking peptide(right).
Rabbit Polyclonal Antibody to Src(Phospho-Tyr529)
-
货号：
P40148 -
别名：
C-SRC; SRC1; -
应用：
WB,IHC,IF -
反应种属：
Human,Mouse,Rat -
抗体类型：
Primary antibody -
Swissprot：
P12931 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Polyclonal Antibody to Src(Phospho-Tyr529)
Description |
|---|
proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. |
References |
|---|
| Pyper J.M., (1985) Mol. Cell. Biol. 5:831-838 Pyper J.M.(1990) Mol. Cell. Biol. 10:2035-2040 Xu W., (1997).Nature 385:595-602 Benes C.H., (2005) Cell 121:271-280 |
Specification |
|
|---|---|
| Aliases | C-SRC; SRC1; |
| Entrez GeneID | 6714; |
| Swissprot | P12931 |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 529 (P-Q-Y(p)-Q-P) derived from Human Src. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50-1/100 |
| IF/ICC | 1/100-1/200 |
Product Image
- Western blot analysis of extracts from HT29 cells untreated or treated with PMA using Src(Phospho-Tyr529) Antibody P40148.
- Western blot analysis of extracts from HepG2 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using Src (Phospho-Tyr529) Antibody P40148.
- Immunofluorescence staining of methanol-fixed Hela cells using Src(Phospho-Tyr529) Antibody P40148.
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src(Phospho-Tyr529) Antibody P40148(left) or the same antibody preincubated with blocking peptide(right).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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