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Western blot analysis of extracts from HT29 cells untreated or treated with PMA using Src(Phospho-Tyr529) Antibody P40148.
Western blot analysis of extracts from HepG2 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using Src (Phospho-Tyr529) Antibody P40148.
Immunofluorescence staining of methanol-fixed Hela cells using Src(Phospho-Tyr529) Antibody P40148.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src(Phospho-Tyr529) Antibody P40148(left) or the same antibody preincubated with blocking peptide(right).

Rabbit Polyclonal Antibody to Src(Phospho-Tyr529)

  • 货号:

    P40148
  • 别名:

    C-SRC; SRC1;
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,IF
  • 反应种属:

    Human,Mouse,Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P12931
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Polyclonal Antibody to Src(Phospho-Tyr529)

Description

proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.

References

Pyper J.M., (1985) Mol. Cell. Biol. 5:831-838 Pyper J.M.(1990) Mol. Cell. Biol. 10:2035-2040 Xu W., (1997).Nature 385:595-602 Benes C.H., (2005) Cell 121:271-280

Specification

Aliases C-SRC; SRC1;
Entrez GeneID 6714;
Swissprot P12931
WB Predicted band size 60kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat
Immunogen Peptide sequence around phosphorylation site of tyrosine 529 (P-Q-Y(p)-Q-P) derived from Human Src.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/500-1/1000
IHC 1/50-1/100
IF 1/100-1/200