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Western blot analysis of extracts from COLO205 cells using cofilin(Phospho-Ser3) Antibody P40135(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Lane 1 : HelaΒ treated with H2O2
Lane 2 : Mouse brain tissue
Lane 3 : Rat brain tissue
Lane 4 : N-peptide blocked HelaΒ treated with H2O2
Lane 5 : N-peptide blocked Mouse brain tissue
Lane 6 : N-peptide blocked Rat brain tissue
Lane 7 : P-peptide blocked HelaΒ treated with H2O2
Lane 8 : P-peptide blocked Mouse brain tissue
Lane 9 : P-peptide blocked Rat brain tissue
Lysates/proteins at 40 μg per lane
Predicted band size :Β 19 kDa
Observed band size :Β 19 kDa
Immunofluorescence staining of methanol-fixed Hela cells using cofilin(Phospho-Ser3) Antibody P40135.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin(Phospho-Ser3) Antibody P40135(left) or the same antibody preincubated with blocking peptide(right).
Rabbit Polyclonal Antibody to cofilin(Phospho-Ser3)
-
货号：
P40135 -
别名：
CFL; CFL1; -
应用：
WB,IHC,IF -
反应种属：
Human,Mouse,Rat -
抗体类型：
Primary antibody -
Swissprot：
P23528 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Polyclonal Antibody to cofilin(Phospho-Ser3)
Description |
|---|
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. |
References |
|---|
| Kobayashi M, et al. (2006) EMBO J 25(4): 713-26. Wang Y, et al. (2005) Biol Chem 280(13): 12683-9. Smith-Beckerman DM, et al. (2005) Mol Cell Proteomics: 156-68. |
Specification |
|
|---|---|
| Aliases | CFL; CFL1; |
| Entrez GeneID | 1072; |
| Swissprot | P23528 |
| WB Predicted band size | 19kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V) derived from Human cofilin. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50-1/100 |
| IF | 1/100-1/200 |
Product Image
- Western blot analysis of extracts from COLO205 cells using cofilin(Phospho-Ser3) Antibody P40135(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
- Lane 1 : HelaΒ treated with H2O2 Lane 2 : Mouse brain tissue Lane 3 : Rat brain tissue Lane 4 : N-peptide blocked HelaΒ treated with H2O2 Lane 5 : N-peptide blocked Mouse brain tissue Lane 6 : N-peptide blocked Rat brain tissue Lane 7 : P-peptide blocked HelaΒ treated with H2O2 Lane 8 : P-peptide blocked Mouse brain tissue Lane 9 : P-peptide blocked Rat brain tissue Lysates/proteins at 40 μg per lane Predicted band size :Β 19 kDa Observed band size :Β 19 kDa
- Immunofluorescence staining of methanol-fixed Hela cells using cofilin(Phospho-Ser3) Antibody P40135.
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin(Phospho-Ser3) Antibody P40135(left) or the same antibody preincubated with blocking peptide(right).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P40135
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