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Western blot analysis of Androgen Receptor(Phospho-Ser213) expression in HEK293T (A), A549 (B), H1792 (C) whole cell lysates.

Immunofluorescent analysis of Androgen Receptor(Phospho-Ser213) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Immunohistochemical analysis of Androgen Receptor(Phospho-Ser213) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Rabbit Polyclonal Antibody to Androgen Receptor(Phospho-Ser213)

货号：
P40117
别名：
ANDR; DHTR; AR
产品上架时间：
2024-01-01
应用：
WB,IHC,IF
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
P10275
规格：

100μl
50μl
数量：

-

+
说明书：

目录价¥2180

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Rabbit Polyclonal Antibody to Androgen Receptor(Phospho-Ser213)

Description
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.

Description

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.

References
Brinkman, A.O. et al. (1999) J. Steroid. Biochem. Mol. Biol. 69, 307-313. Avila, D.M. et al. (2001) J. Steroid. Biochem. Mol. Biol. 76, 135-142. Montgomery, J.S. et al. (2001) J. Pathol. 195, 138-146.

Specification
Aliases	ANDR; DHTR; AR
Entrez GeneID	367;
Swissprot	P10275
WB Predicted band size	110kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Peptide sequence around phosphorylation site of serine 213 (E-A-S(p)-G-A) derived from Human Androgen Receptor.
Formulation	Purified antibody in PBS with 0.05% sodium azide.