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All lanes: Anti-IL6 Antibody (Center) at 1:1000 dilution Lane 1: U-87 MG whole cell lysate Lane 2: Human plasm lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 24KDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-IL6 Antibody (Center) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: NCI-H460 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
IL6 Antibody (Center) (Cat. #P35113) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IL6 Antibody (Center) (P35113)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IL6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Rabbit Polyclonal Antibody to IL6

  • 货号:

    P35113
  • 别名:

    Interleukin-6, IL-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2, IL6, IFNB2
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,FCM
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P05231
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1980

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Rabbit Polyclonal Antibody to IL6

Description

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis.

Specification

Aliases Interleukin-6, IL-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2, IL6, IFNB2
Entrez GeneID 3569
Swissprot P05231
WB Predicted band size 23.7kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This IL6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-55 amino acids from the Central region of human IL6.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/1000
IHC 1/100-1/500
FCM 1/10-1/50