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All lanes : Anti-SCUBE2 Antibody (Center N266) at 1:1000 dilution Lane 1: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 90kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of lysates from Hela, ZR-75-1, HT-29 cell line (from left to right), using SCUBE2 Antibody (Center N266)(Cat. #P34924). P34924 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
SCUBE2 Antibody (Center N266) (Cat. #P34924) western blot analysis in MDA-MB453,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the SCUBE2 antibody detected the SCUBE2 protein (arrow).
Confocal immunofluorescent analysis of SCUBE2 Antibody (Center N266)(Cat#P34924) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
SCUBE2 Antibody (Center N266) (Cat. #P34924)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SCUBE2 Antibody (Center N266) for immunohistochemistry. Clinical relevance has not been evaluated.

Rabbit Polyclonal Antibody to SCUBE2 (Center N266)

  • 货号:

    P34924
  • 别名:

    Signal peptide, CUB and EGF-like domain-containing protein 2, Protein CEGP1, SCUBE2, CEGP1
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,IF
  • 反应种属:

    Human, Mouse, Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q9NQ36
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1980

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Rabbit Polyclonal Antibody to SCUBE2 (Center N266)

Specification

Aliases Signal peptide, CUB and EGF-like domain-containing protein 2, Protein CEGP1, SCUBE2, CEGP1
Entrez GeneID 57758
Swissprot Q9NQ36
WB Predicted band size 110.0kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This SCUBE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-280 amino acids from the Central region of human SCUBE2.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/1000
IHC 1/100-1/500
IF 1/10-1/50

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#P34924