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All lanes : Anti-TUBB2B Antibody (N-term) at 1:4000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: MDA-MB-231 whole cell lysate
Lane 4: K562 whole cell lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Mouse cerebellum tissue lysate
Lane 7: PC-12 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 50 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-TUBB2B Antibody (N-term) at 1:4000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: MDA-MB-231 whole cell lysate
Lane 4: K562 whole cell lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Mouse cerebellum tissue lysate
Lane 7: PC-12 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 50 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-TUBB2B Antibody (N-term) at 1:4000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: MDA-MB-231 whole cell lysate
Lane 4: K562 whole cell lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Mouse cerebellum tissue lysate
Lane 7: PC-12 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 50 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis of paraffin-embedded Human brain tissue using P34685 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:2000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded Human brain tissue using P34685 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:2000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded Human brain tissue using P34685 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:2000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Rabbit Polyclonal Antibody to TUBB2B (N-term)
-
货号：
P34685 -
别名：
Tubulin beta-2B chain, TUBB2B -
应用：
WB,IHC -
反应种属：
Human, Mouse, Rat -
抗体类型：
Primary antibody -
Swissprot：
Q9BVA1 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to TUBB2B (N-term)
Description |
|---|
The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. This gene is highly similar to TUBB2A and TUBB2C. Defects in this gene are a cause of asymmetric polymicrogyria. [provided by RefSeq]. |
Specification |
|
|---|---|
| Aliases | Tubulin beta-2B chain, TUBB2B |
| Entrez GeneID | 347733 |
| Swissprot | Q9BVA1 |
| WB Predicted band size | 50.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This TUBB2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human TUBB2B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/2000 |
| IHC | 1/2000 |
Product Image
- All lanes : Anti-TUBB2B Antibody (N-term) at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Mouse cerebellum tissue lysate Lane 7: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- All lanes : Anti-TUBB2B Antibody (N-term) at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Mouse cerebellum tissue lysate Lane 7: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- All lanes : Anti-TUBB2B Antibody (N-term) at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Mouse cerebellum tissue lysate Lane 7: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Immunohistochemical analysis of paraffin-embedded Human brain tissue using P34685 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:2000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
- Immunohistochemical analysis of paraffin-embedded Human brain tissue using P34685 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:2000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
- Immunohistochemical analysis of paraffin-embedded Human brain tissue using P34685 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:2000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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