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Anti-Sestrin-3 Antibody (Center Y335) at 1:2000 dilution + LNCaP whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 57 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-Sestrin-3 Antibody (Center Y335) at 1:2000 dilution + LNCaP whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 57 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Sestrin-3 Antibody (Center Y335) (Cat. #P34641) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Sestrin-3 antibody detected the Sestrin-3 protein (arrow).
Sestrin-3 Antibody (Center Y335) (Cat. #P34641)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Sestrin-3 Antibody (Center Y335) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Polyclonal Antibody to Sestrin-3 (Center Y335)
-
货号：
P34641 -
别名：
Sestrin-3, SESN3, SEST3 -
应用：
WB,IHC -
反应种属：
Human, Mouse -
抗体类型：
Primary antibody -
Swissprot：
P58005 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to Sestrin-3 (Center Y335)
Description |
|---|
Sestrins form a small family of redox enzymes which regulate accumulation of Reactive Oxygen Species. They protect cells and their DNA against oxidative damage and regulate cell growth and viability. These genes are often deregulated in cancers and their inactivation accelerates the growth of model tumors in mice. |
Specification |
|
|---|---|
| Aliases | Sestrin-3, SESN3, SEST3 |
| Entrez GeneID | 143686 |
| Swissprot | P58005 |
| WB Predicted band size | 57.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Sestrin-3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 320-347 amino acids from the Central region of human Sestrin-3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IHC | 1/100-1/500 |
Product Image
- Anti-Sestrin-3 Antibody (Center Y335) at 1:2000 dilution + LNCaP whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Anti-Sestrin-3 Antibody (Center Y335) at 1:2000 dilution + LNCaP whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Sestrin-3 Antibody (Center Y335) (Cat. #P34641) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Sestrin-3 antibody detected the Sestrin-3 protein (arrow).
- Sestrin-3 Antibody (Center Y335) (Cat. #P34641)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Sestrin-3 Antibody (Center Y335) for immunohistochemistry. Clinical relevance has not been evaluated.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P34641
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