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All lanes : Anti-FBXL2 Antibody (C-term) at 1:1000-1:2000 dilution Lane 1: Human heart lysate Lane 2: Human liver lysate Lane 3: Hela whole cell lysate Lane 4: MDA-MB-453 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
FBXL2 Antibody (C-term) (Cat. #P34612) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
FBXL2 Antibody (C-term) (Cat. #P34612) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FBXL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Rabbit Polyclonal Antibody to FBXL2

  • 货号:

    P34612
  • 别名:

    F-box/LRR-repeat protein 2, F-box and leucine-rich repeat protein 2 {ECO:0000312|HGNC:HGNC:13598}, F-box protein FBL2/FBL3 {ECO:0000303|PubMed:10945468, ECO:0000312|EMBL:AAF045101}, FBXL2 (HGNC:13598)
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,FCM
  • 反应种属:

    Human, Mouse, Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q9UKC9
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1980

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Rabbit Polyclonal Antibody to FBXL2

Description

FBXL2 encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats.

Specification

Aliases F-box/LRR-repeat protein 2, F-box and leucine-rich repeat protein 2 {ECO:0000312|HGNC:HGNC:13598}, F-box protein FBL2/FBL3 {ECO:0000303|PubMed:10945468, ECO:0000312|EMBL:AAF045101}, FBXL2 (HGNC:13598)
Entrez GeneID 25827
Swissprot Q9UKC9
WB Predicted band size 47.1kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This FBXL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-395 amino acids from the C-terminal region of human FBXL2.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/1000
IHC 1/100-1/500
FCM 1/10-1/50