搜索
请登录
提醒成功
微信/QQ登录
搜索
首页
Anti-SPNS2 Antibody (N-term) at 1:1000 dilution + SK-OV-3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 58 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-SPNS2 Antibody (N-term) at 1:1000 dilution with human heart tissue lysate Lysates/proteins at 20 µg per lane.
Overlay histogram showing SK-OV-3 cells stained with P34593(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Immunohistochemical analysis of SPNS2 on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Rabbit Polyclonal Antibody to SPNS2 (N-term)
-
货号：
P34593 -
别名：
Protein spinster homolog 2, SPNS2 -
应用：
WB,FCM,IHC-P -
反应种属：
Human, Mouse -
抗体类型：
Primary antibody -
Swissprot：
Q8IVW8 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to SPNS2 (N-term)
Description |
|---|
Sphingolipid transporter required for migration of myocardial precursors. Transports sphingosine 1-phosphate (S1P), a secreted lipid mediator that plays critical roles in cardiovascular, immunological, and neural development and function. Mediates the export of S1P from cells in the extraembryonic yolk syncytial layer (YSL), thereby regulating myocardial precursor migration. |
Specification |
|
|---|---|
| Aliases | Protein spinster homolog 2, SPNS2 |
| Entrez GeneID | 124976 |
| Swissprot | Q8IVW8 |
| WB Predicted band size | 58.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This SPNS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-94 amino acids from the N-terminal region of human SPNS2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/50-1/100 |
| FCM | 1/25 |
Product Image
- Anti-SPNS2 Antibody (N-term) at 1:1000 dilution + SK-OV-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Anti-SPNS2 Antibody (N-term) at 1:1000 dilution with human heart tissue lysate Lysates/proteins at 20 µg per lane.
- Overlay histogram showing SK-OV-3 cells stained with P34593(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
- Immunohistochemical analysis of SPNS2 on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P34593
相关产品
联系方式
CONTACT-
联系电话:
0731-88388787 -
公司邮箱:
sales@promab.cn -
公司地址:
湖南省长沙市高新开发区林语路239号顺畅产业园5楼
湖南省长沙市望城区普瑞大道8号出版科技园2号楼
官方微信
产品中心
Product技术服务
service客户留言
message