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Anti-POTEE Antibody (Center) at 1:1000 dilution + LNCap whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 121 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing K562 cells stained with P34583(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34583, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Rabbit Polyclonal Antibody to POTEE
-
货号：
P34583 -
别名：
POTE ankyrin domain family member E, ANKRD26-like family C member 1A, Prostate, ovary, testis-expressed protein on chromosome 2, POTE-2, POTEE, A26C1A, POTE2 -
应用：
WB,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q6S8J3 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to POTEE
Specification |
|
|---|---|
| Aliases | POTE ankyrin domain family member E, ANKRD26-like family C member 1A, Prostate, ovary, testis-expressed protein on chromosome 2, POTE-2, POTEE, A26C1A, POTE2 |
| Entrez GeneID | 445582 |
| Swissprot | Q6S8J3 |
| WB Predicted band size | 121.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This POTEE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-409 amino acids from the Central region of human POTEE. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
| FCM | 1/25 |
Product Image
- Anti-POTEE Antibody (Center) at 1:1000 dilution + LNCap whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 121 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Overlay histogram showing K562 cells stained with P34583(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34583, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P34583
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