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Anti-SEC24B Antibody (C-Term) at 1:2000 dilution + 293 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 137 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing A431 cells stained with P34509(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Rabbit Polyclonal Antibody to SEC24B
-
货号：
P34509 -
别名：
Protein transport protein Sec24B, SEC24-related protein B, SEC24B -
应用：
WB,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
O95487 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to SEC24B
Description |
|---|
Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex. |
Specification |
|
|---|---|
| Aliases | Protein transport protein Sec24B, SEC24-related protein B, SEC24B |
| Entrez GeneID | 10427 |
| Swissprot | O95487 |
| WB Predicted band size | 137.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SEC24B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1209-1239 amino acids from the human region of human SEC24B. |
Application |
|
|---|---|
| WB | 1/2000 |
| FCM | 1/25 |
Product Image
- Anti-SEC24B Antibody (C-Term) at 1:2000 dilution + 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 137 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Overlay histogram showing A431 cells stained with P34509(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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