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All lanes : Anti-ADD1 Antibody (Center) at 1:2000 dilution
Lane 1: 293 whole cell lysate
Lane 2: HT-29 whole cell lysate
Lane 3: U-2 OS whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 81 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing A431 cells stained with P34503(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Rabbit Polyclonal Antibody to ADD1
-
货号：
P34503 -
别名：
Alpha-adducin, Erythrocyte adducin subunit alpha, ADD1, ADDA -
应用：
WB,FCM -
反应种属：
Human, Rat -
抗体类型：
Primary antibody -
Swissprot：
P35611 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to ADD1
Description |
|---|
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. |
Specification |
|
|---|---|
| Aliases | Alpha-adducin, Erythrocyte adducin subunit alpha, ADD1, ADDA |
| Entrez GeneID | 118 |
| Swissprot | P35611 |
| WB Predicted band size | 81.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | This ADD1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 428-462 amino acids from the Central region of human ADD1. |
Application |
|
|---|---|
| WB | 1/2000 |
| FCM | 1/25 |
Product Image
- All lanes : Anti-ADD1 Antibody (Center) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: U-2 OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 81 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Overlay histogram showing A431 cells stained with P34503(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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