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All lanes : Anti-hBik-BH3 at 1:2000 dilution
Lane 1: A431 whole cell lysate
Lane 2: Ramos whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 18 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Bik Antibody (BH3) (Cat. #P34497) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Bik antibody detected the Bik protein (arrow).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Polyclonal Antibody to Bik BH3 Domain
-
货号：
P34497 -
别名：
Bcl-2-interacting killer, Apoptosis inducer NBK, BIP1, BP4, BIK, NBK -
应用：
WB,IHC -
反应种属：
Human, Mouse -
抗体类型：
Primary antibody -
Swissprot：
Q13323 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to Bik BH3 Domain
Description |
|---|
The Bik protein is known to interact with cellular and viral survival-promoting proteins, such as BCL2 and the Epstein-Barr virus in order to enhance programmed cell death. Because its activity is suppressed in the presence of survival-promoting proteins, this protein is suggested as a likely target for antiapoptotic proteins. This protein shares a critical BH3 domain with other death-promoting proteins, BAX and BAK. |
Specification |
|
|---|---|
| Aliases | Bcl-2-interacting killer, Apoptosis inducer NBK, BIP1, BP4, BIK, NBK |
| Entrez GeneID | 638 |
| Swissprot | Q13323 |
| WB Predicted band size | 18.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Bik BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-74 amino acids from human Bik BH3 Domain. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IHC | 1/100-1/500 |
Product Image
- All lanes : Anti-hBik-BH3 at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Ramos whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 18 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Bik Antibody (BH3) (Cat. #P34497) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Bik antibody detected the Bik protein (arrow).
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P34497
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