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Overlay histogram showing PHA-stimulated huuman peripheral blood lymphocytes stained with CD25 antibody(green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:50 dilution) for 60min at 37ºC. The secondary antibody used was Goat Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40min at 37ºC. Isotype control antibody (blue line) was mouse IgG2a (1 µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Mouse Monoclonal Antibody to human CD25
-
货号：
P34492 -
别名：
-
应用：
FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
unknow -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to human CD25
Specification |
|
|---|---|
| Swissprot | unknow |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Formulation | Purified antibody in TBS with 0.05% sodium azide. |
Application |
|
|---|---|
| FCM | 1/50 |
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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