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Anti-T Antibody (N-term) at 1:2000 dilution + NCI-H460 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized A549 cells labeling TBXT with P34475 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus, cytoplasm weakly staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/500 dilution (red). The nuclear counter stain is DAPI (blue).

Rabbit Polyclonal Antibody to T (N-term)

货号：
P34475
别名：
Brachyury protein, Protein T, T
产品上架时间：
2024-01-01
应用：
WB,IF
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
O15178
规格：

100μl
50μl
数量：

-

+
说明书：

目录价¥1980

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Rabbit Polyclonal Antibody to T (N-term)

Description
The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

Specification
Aliases	Brachyury protein, Protein T, T
Entrez GeneID	6862
Swissprot	O15178
WB Predicted band size	47.4kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	This T antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human T.
Formulation	Purified antibody in PBS with 0.05% sodium azide.