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Anti-DSPP Antibody (N-term) at 1:2000 dilution + PC-3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 131 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing PC-3 cells stained with P34428(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Rabbit Polyclonal Antibody to DSPP (N-term)
-
货号：
P34428 -
别名：
Dentin sialophosphoprotein, Dentin phosphoprotein, Dentin phosphophoryn, DPP, Dentin sialoprotein, DSP, DSPP -
应用：
WB,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q9NZW4 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to DSPP (N-term)
Description |
|---|
This gene encodes two principal proteins of the dentin extracellular matrix of the tooth. The preproprotein is secreted by odontoblasts and cleaved into dentin sialoprotein and dentin phosphoprotein. Dentin phosphoprotein is thought to be involved in the biomineralization process of dentin. Mutations in this gene have been associated with dentinogenesis imperfecta-1; in some individuals, dentinogenesis imperfecta occurs in combination with an autosomal dominant form of deafness. Allelic differences due to repeat polymorphisms have been found for this gene. [provided by RefSeq]. |
Specification |
|
|---|---|
| Aliases | Dentin sialophosphoprotein, Dentin phosphoprotein, Dentin phosphophoryn, DPP, Dentin sialoprotein, DSP, DSPP |
| Entrez GeneID | 1834 |
| Swissprot | Q9NZW4 |
| WB Predicted band size | 131.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This DSPP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-76 amino acids of human DSPP. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/2000 |
| FCM | 1/25 |
Product Image
- Anti-DSPP Antibody (N-term) at 1:2000 dilution + PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 131 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Overlay histogram showing PC-3 cells stained with P34428(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P34428
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