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All lanes : Anti-COXI Antibody (Center) at 1:2000 dilution
Lane 1: human heart lysate
Lane 2: mouse spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 57 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling COXI with P34409 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and mitochondrion staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
Rabbit Polyclonal Antibody to COXI
-
货号：
P34409 -
别名：
Cytochrome c oxidase subunit 1, Cytochrome c oxidase polypeptide I, MT-CO1, COI, COXI, MTCO1 -
应用：
WB,IF -
反应种属：
Human, Mouse, Rat -
抗体类型：
Primary antibody -
Swissprot：
P00395 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to COXI
Specification |
|
|---|---|
| Aliases | Cytochrome c oxidase subunit 1, Cytochrome c oxidase polypeptide I, MT-CO1, COI, COXI, MTCO1 |
| Entrez GeneID | 4512 |
| Swissprot | P00395 |
| WB Predicted band size | 57.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This COXI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-224 amino acids of human COXI. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/2000 |
| IF | 1/25 |
Product Image
- All lanes : Anti-COXI Antibody (Center) at 1:2000 dilution Lane 1: human heart lysate Lane 2: mouse spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling COXI with P34409 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and mitochondrion staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P34409
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