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All lanes : Anti-DBH Antibody (N-term P42) at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: mouse adrenal gland lysate Lane 3: rat adrenal gland lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling DBH with P34386 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and weak nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

Rabbit Polyclonal Antibody to DBH (N-term P42)

  • 货号:

    P34386
  • 别名:

    Dopamine beta-hydroxylase, Dopamine beta-monooxygenase, Soluble dopamine beta-hydroxylase, DBH
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IF
  • 反应种属:

    Human, Mouse, Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P09172
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1980

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Rabbit Polyclonal Antibody to DBH (N-term P42)

Description

The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq].

Specification

Aliases Dopamine beta-hydroxylase, Dopamine beta-monooxygenase, Soluble dopamine beta-hydroxylase, DBH
Entrez GeneID 1621
Swissprot P09172
WB Predicted band size 69.1kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This DBH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 amino acids from the N-terminal region of human DBH.
Formulation Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) .

Application

WB 1/2000
IF 1/25