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All lanes : Anti-EEF1A1P5 Antibody (C-Term) at 1:1000-1:2000 dilution Lane 1: U-2OS whole cell lysate Lane 2: 293 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling EEF1A1P5 with P34361 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

Overlay histogram showing HepG2 cells stained with P34361(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34361, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. .

Rabbit Polyclonal Antibody to EEF1A1P5

货号：
P34361
别名：
Putative elongation factor 1-alpha-like 3, EF-1-alpha-like 3, Eukaryotic elongation factor 1 A-like 3, eEF1A-like 3, Eukaryotic translation elongation factor 1 alpha-1 pseudogene 5, EEF1A1P5, EEF1AL3
产品上架时间：
2024-01-01
应用：
WB,IF,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
Q5VTE0
规格：

100μl
50μl
数量：

-

+
说明书：

目录价¥1980

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Rabbit Polyclonal Antibody to EEF1A1P5

Description
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Specification
Aliases	Putative elongation factor 1-alpha-like 3, EF-1-alpha-like 3, Eukaryotic elongation factor 1 A-like 3, eEF1A-like 3, Eukaryotic translation elongation factor 1 alpha-1 pseudogene 5, EEF1A1P5, EEF1AL3
Swissprot	Q5VTE0
WB Predicted band size	50.2kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This EEF1A1P5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 430-462 amino acids from human EEF1A1P5.