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All lanes : Anti-IGF2BP1 Antibody (C-term) at 1:1000-1:2000 dilution Lane 1: NIH/3T3 whole cell lysate Lane 2: 293 whole cell lysate Lane 3: RD whole cell lysate Lane 4: Hela whole cell lysate Lane 5: HepG2 whole cell lysate Lane 6: Jurkat whole cell lysate Lane 7: Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 63 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing HepG2 cells stained with P34349(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34349, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Rabbit Polyclonal Antibody to IGF2BP1

货号：
P34349
别名：
Insulin-like growth factor 2 mRNA-binding protein 1, IGF2 mRNA-binding protein 1, IMP-1, IMP1, Coding region determinant-binding protein, CRD-BP, IGF-II mRNA-binding protein 1, VICKZ family member 1, Zipcode-binding protein 1, ZBP-1, IGF2BP1, CRDBP, VICKZ1, ZBP1
产品上架时间：
2024-01-01
应用：
WB,FCM
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
Q9NZI8
规格：

100μl
50μl
数量：

-

+
说明书：

目录价¥1980

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Rabbit Polyclonal Antibody to IGF2BP1

Description
IGF2BP1 is a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene.

Description

IGF2BP1 is a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene.

Specification
Aliases	Insulin-like growth factor 2 mRNA-binding protein 1, IGF2 mRNA-binding protein 1, IMP-1, IMP1, Coding region determinant-binding protein, CRD-BP, IGF-II mRNA-binding protein 1, VICKZ family member 1, Zipcode-binding protein 1, ZBP-1, IGF2BP1, CRDBP, VICKZ1, ZBP1
Entrez GeneID	10642
Swissprot	Q9NZI8
WB Predicted band size	63.5kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This IGF2BP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 508-534 amino acids from the C-terminal region of human IGF2BP1.
Formulation	Purified antibody in PBS with 0.05% sodium azide.